Roof Studio Hostel Rethymno lies in Old Town Rethymno with its maze of cobblestone alleyways lined with museums, cozy tavernas, and restaurants offering traditional specialties, and the nearest bus stop, Emmanoil Portaliou 188-200, is just 0.8 km away.

Located around a 5-minute drive from such significant cultural landmarks as the 17th - century Venetian Rimondi Fountain, the hostel is also within 20 minutes' walk of the venetian fortress "Fortezza of Rethymno". Guests can explore nearby places of worship, including Savior Monastery, which is 2.4 km from the Rethymno hostel. The well-curated Archaeological Museum of Rethymno is within a few blocks of the accommodation.

The restaurant La Boheme is in the immediate vicinity of Roof Studio hostel.